Due to the exhaustion of cell-free gene expression systems over time, it is difficult to realize cell-free gene circuits in closed reactors that require extended runtimes for operation. In this work we show that execution of an incoherent feed-forward loop of type 2 (IFFL-2) - capable of distinguishing between high, intermediate and low input signals - can be successfully established in microfluidic ring reactors, which allow running the circuit under steady state conditions.
A.C. Jäkel, L. Aufinger, F.C. Simmel, Steady-State Operation of a Cell-Free Genetic Band-Detection Circuit, ACS Synth Biol. 2022 Sep 12. doi: 10.1021/acssynbio.2c00195. Epub ahead of print.