PylD holoenzyme from M. barkeri

[100] Quitterer F., Beck P., Bacher A., Groll M.
Structure and reaction mechanism of pyrrolysine synthase (PylD)
Angew. Chem. Int. Ed., 2013, 52, 7033-7, PDF

The final step in the biosynthesis of the 22nd genetically encoded amino acid, pyrrolysine, is catalyzed by PylD, a structurally and mechanistically unique dehydrogenase. This catalyzed reaction includes an induced-fit mechanism achieved by major structural rearrangements of the N-terminal helix upon substrate binding. Different steps of the reaction trajectory are visualized by complex structures of PylD with substrate and product.