Publications
2025
- Enhancing Enzyme Stability for Biotechnological Applications
Benítez-Mateos, A. I.
CHIMIA, 2025, 79: 792-796. - Enhancement of enzymatic activity by biomolecular condensates through pH buffering
Stoffel, F., Papp, M., Gil-Garcia, M., Küffner, A. M. , Benítez-Mateos, A. I., Jacquat, R. P. B., Galvanetto, N., Faltova, L., Arosio, P.
Nature Communications, 2025, 16: 6368. - Pioneering Women Shaping the Field of Biocatalysis
Benítez-Mateos, A. I., Paul, C. E., Schmidt, S., Contente, M. L.
ChemCatChem, 2025, 17: e202500088. - Biocatalytic Reduction of Heterocyclic Imines in Continuous Flow with Immobilized Enzymes
Benítez-Mateos, A. I., Lim, D., Roura Padrosa, D., Marchini, V., Wu, H., Buono, F., Paradisi, F.
ACS Sustainable Chemistry & Engineering, 2025, 13: 5009-5018.
2024
- Optimized Spatial Configuration of Heterogeneous Biocatalysts Maximizes Cell-Free Biosynthesis of ω-Hydroxy and ω-Amino Acids
Santiago-Arcos, J., Velasco-Lozano, S., Diamanti, E., Benítez-Mateos, A. I., Grajales-Hernández, D., Paradisi, F., López-Gallego, F.
ACS Sustainable Chemistry & Engineering, 2024, 12: 9474-9489. - Towards Greener and More Cost-efficient Biosynthesis of Pharmaceuticals and Fragrance Molecules
Benítez-Mateos, A. I.*
CHIMIA, 2024, 78: 222-225. - Local environment in biomolecular condensates modulates enzymatic activity across length scales
Gil-Garcia, M., Benítez-Mateos, A. I., Papp, M., Stoffel, F., Morelli, C., Normak, K., Makasewicz, K., Faltova, L., Paradisi, F., Arosio, P.
Nature Communications, 2024, 15: 3322. - Biocatalytic Synthesis of L-Pipecolic Acid by a Lysine Cyclodeaminase: Batch and Flow Reactors
Stalder, K., Benítez-Mateos, A. I.*, Paradisi, F.*
ChemCatChem, 2024, e202301671. - Perspectives on Flow Biocatalysis: The Engine Propelling Enzymatic Reactions
Benítez-Mateos, A. I.*, Paradisi, F.*
Journal of Flow Chemistry, 2024, 14: 211-218.
2023
- Halomonas elongata: a microbial source of highly stable enzymes for applied biotechnology
Benítez-Mateos, A. I.*, Paradisi, F.*
Applied Microbiology and Biotechnology. 2023, 107: 3183-3190.
2022
- Back to the future: taking enzymes to the next level of sustainability
Roura Padrosa, D., Benítez-Mateos, A. I., Paradisi, F.
The Biochemist. 2022, 49: 19-22. - Spheroplast preparation boosts the catalytic potential of a squalene-hopene cyclase
Benítez-Mateos, A. I., Schneider, A., Hegarty, E., Hauer, B., Paradisi, F.
Nature Communications. 2022, 13: 6269. - Novel triple mutant of an extremophilic glycosyl hydrolase enables the rapid synthesis of thioglycosides
Pillet, L., Lim, D., Almulhim, N., Benítez-Mateos, A. I., Paradisi, F.
Chemical Communications. 2022, 58: 12118-12121. - Fusion of formate dehydrogenase and alanine dehydrogenase as an amino donor regenerating system coupled to transaminases
Marchini, V., Benítez-Mateos, A. I., Hutter, S. L., Paradisi, F.
ChemBioChem. 2022, 22: e202200428. - A novel thymidine phosphorylase to synthesize (halogenated) anticancer and antiviral nucleoside drugs in continuous flow
Benítez-Mateos, A. I., Klein, C., Roura Padrosa, D., Paradisi, F.
Catalysis Science and Technology. 2022, 12: 6231-6238 - Multi-step enzyme cascades as a route towards green and sustainable pharmaceutical syntheses
Benítez-Mateos, A. I., Roura Padrosa, D., Paradisi, F.
Nature Chemistry. 2022, 14: 489-499. - Metal substrate catalysis in the confined space for platinum drug delivery
Velasco-Lozano, S., Alonso-de Castro, S., Sánchez-Cano, C., Benítez-Mateos, A. I., López-Gallego, F., Salassa, L.
Chemical Science. 2022, 13: 59-67. - Sustainable flow-synthesis of (bulky)nucleoside drugs by a novel and highly stable nucleoside phosphorylase immobilized on reusable supports
Benítez-Mateos, A. I., Paradisi, F.
ChemSusChem. 2022, 15: e2021020.
2021
- Fusion of glutamate dehydrogenase and formate dehydrogenase yields a bifunctional efficient biocatalyst for the continuous removal of ammonia
Marchini, V., Benítez-Mateos, A. I., Roura Padrosa, D., Paradisi, F.
Frontiers in Catalysis. 2021, 1: 790461. - Agarose vs methacrylate as material supports for enzyme immobilisation and continuous processing
Benítez-Mateos, A. I., Contente, M. L.
Catalysts. 2021, 11: 814. - Dual revalorization of lignin through its use as a versatile and renewable matrix for enzyme immobilisation and (flow)bioprocess engineering
Benítez-Mateos, A. I., Bertella, S., De Bueren, J. B., Luterbacher, J., Paradisi, F.
ChemSusChem. 2021, 14: 3198-3207. - Flow biocatalysis 101: design, development and applications
Benítez-Mateos, A. I., Contente, M. L., Roura Padrosa, D., Paradisi, F.
Reaction Chemistry and Engineering. 2021, 6: 599-611 - Solid phase assembly of multi-enzyme systems into artificial cellulosomes
Zeballos, N., Diamanti, E., Benítez-Mateos, A. I., Schmidt-Dannert, C., López-Gallego, F.
Bioconjugate Chemistry. 2021, 9: 2971-2978.
2020
- Cell-free biocatalytic syntheses of L-pipecolic acid: a dual strategy approach and process intensification in flow
Roura Padrosa, D.,# Benítez-Mateos, A. I., # Calvey, L., Paradisi, F.
Green Chemistry. 2020, 22: 5310-5316. - Micro-compartmentalized cell-free protein synthesis in hydrogel μ-channels
Benítez-Mateos, A. I.,* Zeballos, N., Comino, N., Moreno-de-Redrojo, L., Randelovic, T., López-Gallego, F.*
ACS Synthetic Biology. 2020, 9: 2971-2978. - Design of the enzyme-carrier interface to overcome the O2 and NADH mass transfer limitations of an immobilized flavin-oxidase
Benítez-Mateos, A. I., Huber, C., Nidetzky, B., Bolivar, J. M., López-Gallego, F.
ACS Applied Materials & Interfaces. 2020, 12: 56027-56038.
2019
- Deciphering the effect of microbead size distribution on the kinetics of heterogeneous biocatalysts through single-particle analysis based on fluorescence microscopy
Muñoz-Morales, E., Velasco-Lozano, S., Benítez-Mateos, A. I., Marín, M. J., Ramos-Cabrer, P., López-Gallego, F.
Catalysts. 2019, 9: 896. - Selective immobilisation of fluorescent proteins for the fabrication of photoactive materials
Benítez-Mateos, A. I., Mehravar, E., Velasco-Lozano, S., Tomovska, R., Salassa, L., López-Gallego, F.
Molecules. 2019, 24: 2775.
2018
- Self-sufficient flow-biocatalysis by coimmobilisation of pyridoxal 5′-phosphate and ω-transaminases onto porous carriers
Benítez-Mateos, A. I., Contente, M. L., Velasco-Lozano, S., Paradisi, F., López-Gallego, F.
ACS Sustainable Chemistry & Engineering. 2018, 6: 13151-13159. - Expanding one-pot cell-free protein synthesis and immobilisation for on-demand manufacturing of biomaterials
Benítez-Mateos, A. I., Llarena, I., Sánchez-Iglesias, A., López-Gallego, F.
ACS Synthetic Biology. 2018, 7: 875-884. - Chemoenzymatic approaches to the synthesis of the calcimimetic agent cinacalcet employing transaminases and ketoreductases
Marx, L., Ríos-Lombardía, N., Farnberger, J., Kroutil, W., Benítez-Mateos, A. I., López-Gallego, F., Morís, F., González-Sabín, J., Berglund, P.
Advanced Synthesis & Catalysis. 2018, 360: 2157-2165.
2017
- Single-particle studies to advance the characterization of heterogeneous biocatalysts
Benítez-Mateos, A. I., Nidetzky, B., Bolivar, J. M., López-Gallego, F.
ChemCatChem. 2017, 10: 654- 665. - Asymmetric reduction of prochiral ketones by using self-sufficient heterogeneous biocatalysts based on NADPH-dependent ketoreductases
Benítez-Mateos, A. I., San Sebastián, E., Ríos-Lombardía, N., Morís, F., González-Sabín, J., López Gallego, F.
Chemistry – A European Journal. 2017, 23: 16843-16852. - Co-immobilized phosphorylated cofactors and enzymes as self-sufficient heterogeneous biocatalysts for chemical processes
Velasco-Lozano, S., Benítez-Mateos, A. I., López-Gallego, F.
Angewandte Chemie International Edition. 2017, 56: 771-775.